Vibrio areninigrae as a pathogenic bacterium in a crustacean

The occurrence of infectious diseases poses a significant threat to the aquaculture industry worldwide. Therefore, characterization potentially harmful pathogens is one most important strategies control disease outbreaks. In present study, we investigated for first time pathogenicity two Vibrio species, metschnikovii, foodborne pathogen that causes fatalities in humans, and areninigrae, bacteria isolated from black sand Korea, using crustacean model, signal crayfish Pacifastacus leniusculus. Mortality challenges indicated injection V. metschnikovii (108 CFU/crayfish) has mortality percentage 22% crayfish. contrast, P. leniusculus with 108 or 107 CFU areninigrae resulted 100% within days post-injection, respectively. was successfully re-isolated hepatopancreas infected caused when reinjected into new healthy As consequence this infection, histopathological analysis revealed nodule formation hepatopancreas, heart, gills, as well sloughed cells inside hepatopancreatic tubules atrophy. Moreover, extracellular crude products (ECP’s) were obtained order investigate putative virulence factors. vivo ECP’s >90% mortalities 24 h. vitro hemocytes induced cytotoxicity hour exposure. These findings represent report highly pathogenic bacterium can cause crustaceans. On contrary, could not freshwater